Introduction
Toxoplasmosis is a disease caused by a single celled parasite called Toxoplasma gondii (T.
gondii). Toxoplasmosis is one of the most common parasitic diseases and has been found in
nearly all warm-blooded animals, including pets and humans. Cats are important in the
epidemiology of T. gondii because they are the only hosts that can excrete environmentally
resistant oocysts. Most cats infected with T. gondii will not show any symptoms. Occasionally,
however, clinical disease toxoplasmosis occurs. When disease does occur, it may develop when
the cat’s immune response is not adequate to stop the spread of tachyzoite forms. The disease is
more likely to occur in cats with suppressed immune systems, including young kittens and cats
with feline leukemia virus (FELV) or feline immunodeficiency virus (FIV).
Principles
Feline Toxo IgG/IgM Ab test kit is for the simultaneous detection of anti-Toxo IgG
and anti-Toxo IgM Ab in feline whole blood, serum or plasma. The test results can appear on
Control (C) and Test (T) lines where the principles of immunochromatography are used.
Characteristics
1) One-step rapid test of Toxoplasma gondii IgG/IgM Ab
2) Rapid test results between 5 ~ 10 minutes
3) Expensive equipment not required
4) Easy storage and maintenance
5) High-purity and high-quality materials of the test kit increase its sensitivity and specificity.
Materials (10 tests/kit)
1) Test --------------------------------------------------------------- 10 units
2) Diluent (buffer) ------------------------------------------------- 3 x 1 unit
3) Disposable dropper for sample collection ------------ 10 units
Composition
Specimen well (S: for dropping), Test line (T), and Control line (C) are marked on the device.
Inside it, the strip is composed of sample pad, conjugate pad, nitrocellulose membrane (test
paper), and absorbent pad
Effect
Simultaneous detection of anti-Toxo IgG and anti-Toxo IgM Ab in Feline whole blood, serum or
plasma
Uses
1) Specimen
Feline whole blood, serum or plasma
2) Test procedure
a) When specimen and test kits are stored at cold circumstances (2~8 °C), put them at
room temperature for 15~30 minutes before use.
b) Take out a device from a pouch and place it on a horizontal surface.
c) Using a dropper as a pipette, obtain the specimen and dispense 1 drop (40 ) of the
fluid into the IgG Ab specimen well(S) and 1 drop (40 ) of the fluid into the IgM Ab
specimen well(S) of appropriately labeled device.
d) When the specimen is completely absorbed into each the specimen well (S), drop
about 1 drop (40 ) of buffer , respectively.
e) Read the test results between 5 ~ 10 minutes.
Consider the test results as invalid after 10 minutes.
3) Interpretation of the results
A purple band should appear on the control line regardless of the test result. The
presence of another band on the test line determines the result.
Control Line (C): The line should always appear. If this line does not appear, the test should
be considered invalid. This is probably because of impure buffer and the lack of specimen. It
should be tested again with new buffer.
Test Line (T): The presence of Toxo IgG and/or Toxo IgM Ab determines the test line.
(1) Toxo IgG Negative / Toxo IgM Negative
(2) Toxo IgG Positive / Toxo IgM Negative
(3) Toxo IgG Negative / Toxo IgM Positive
(4) Toxo IgG Positive / Toxo IgM Positive
(5) Retesting
a) Both Test and Control lines do not appear.
b) Test line only appears.
IgG Positive (+):
An IgG positive result means that cat has infected with T. gondii at most 1 year ago. It may be
previous or chronic infection.
IgG Negative (-):
An IgG negative result indicates that 1) the cat has not been infected or 2) the cat has no IgG
antibody to T. gondii.
IgM Positive (+):
An IgM positive result means that cat has infected with T. gondii at most 4 months ago. It may
be primary infection.
IgM Negative (-):
An IgM negative result indicates that 1) the cat has not been infected or 2) the cat has no IgM
antibody to T. gondii.
Both IgG and IgM Positive (+):
Both IgG and IgM positive result means that cat has infected with T. gondii between 2 weeks
and 4 months. It may be acute infection.
Further examinations
This test is for primary screening. Consult veterinarians for further necessary examinations to
obtain clinical test results.
Precautions
1) Use for feline In-vitro diagnostic purposes only.
2) Use within 10 minutes after opening the pouch because the test kit is very sensitive to
moisture and its effect may diminish.
3) Be careful of not touching the result window.
4) Every specimen should be used with different droppers.
5) For testing, buffer included only should be used.
6) Do not use specimen showing hemolysis or being contaminated with microbes,
which may cause false-positive or negative result.
7) Deal with specimen carefully. They can deliver unknown virus or infectious bacteria.
8) Use disposable gloves when you suspect the infection caused by specimen. And
wash your hands later.
9) Dispose solid wastes after sterilizing them at 121 °C for over 1 hour.
10) Do not the kit use when its pouch is torn, sealing is not good shape and expiration date
passes.
Storage method
The test kits store at 2~30°C can be used for 24 months after manufacturing. Do not keep
them in refrigerator. However, if they are stored under cold circumstances, keep them at room
temperature for 15 ~ 30 minutes before use.
Exchange
The test kits are manufactured under strict quality control system. Nevertheless, if they
deteriorate during delivery, ask our distributors for exchange.
Liability
The entire risk due to the performance of this product is assumed by the purchaser. The
manufacturer shall not be liable for indirect, special or consequential damages of any kind
resulting from use of this product.
